DNA extraction from sediments
PCR
Cloning
Screening of 16S rDNA gene banks
Sequence analysis
Fluorescence in situ hybridisation (FISH)
CARD FISH

DNA extraction from sediments

For the extraction of genomic DNA from environmental samples, we use a protocol modified after Zhou et al. (1996).

Reagents and Solutions:

DNA-extraction buffer: 100 mM Tris-HCl (pH 8.0) 100 mM EDTA (pH 8.0) 100 mM Na-phosphate (pH 8.0) 1.5 mM NaCl 1% CTAB (Hexadecyltrimethylammonium bromide)

10 mg/ml proteinase K

Chloroform-isoamyl alcohol (24:1 v/v)

20% SDS

Isopropanol

80% ethanol

Extraction



(Continue with DNA extraction methods on next page)



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