Fluorescence in situ hybridisation (FISH)

Field

15 ml vials are prepared with 3 ml formaldehyde (4% formaldehyde in seawater)

The sediment is sampled with a 5 ml capped syringe of which 1 ml is transferred to the formaldehyde and vortexed.

Vials are left for 4 h for fixation and well mixed before 2 ml of the suspension is transferred to a 2 ml Eppendorf vial with a cut-off pipette tip.

The Eppendorf vials are centrifuged (max rpm for 5 min) and the supernatant is discharged

Laboratory

Ultrasound (optional)





(continue with FISH methods on next page)



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