Methane oxidation

A tubing connected to the water bottle of the CTD and a 20 ml glass vial are rinsed with the water from the CTD before filling the vial with the water and closing it without trapping gas bubbles. For each sample point 6 water-filled vials are collected (4x sample plus 2x control) and kept at in situ temperature.

The controls are treated with 0.5 ml formaldehyde (37%) which kills cells and stops processes.

6 ml scintillation vials with 4 ml Ultima Gold are prepared and 1 ml of the sample is added. The vial is immediately capped and vortexed.

Measurement in the scintillation counter should take place as soon as possible.

The glass vials with the samples are decapped.

1/4 teespoon NaCl + 0.5 ml formaldehyde (37%) are prepared for each sample in a 20 ml scintillation vial and 5 ml of the sample added.

The scintillation vials are capped, vortexed for 30 sec, and then left open for 1 hour in a fume hood so that the radiotracer can evaporate. This is repeated 3 times.

12 ml Ultima Gold is added and the activity measured again.

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